Effect of mineral exposure on macrophage function.
College of Medicine, University of Arizona, Tucson, Arizona 1988 Dec; :1-30
The effects on individual alveolar macrophages of in-vitro and in- vivo exposures to minerals found associated with coal mine dust were studied. Cells were lavaged from male Long-Evans-hooded-rats. Effects were examined using electrophysiological, electrooptical, and quantitative electron microscopic techniques. The electrooptical technique was used to measure the production of superoxide (SO) anion in individual cells. Membrane electrical activity in stimulated and unstimulated cells was measured by electrophysiological voltage clamp techniques. SO production was used to determine differential effects between in-vitro and in-vivo exposure to silica (7631869). In-vitro exposure to silica resulted in decreases in both the rate and maximum production of SO by the cells, whereas in-vivo exposure produced a population of cells which significantly increased the quantities of SO at 3 and 10 days following a 4 week exposure. A synthetic coal mine dust significantly increased the amount of SO produced following in-vitro exposure and resulted in significant increases in the amount of SO produced and the rate of production at 10 days, and 10 and 31 days after in-vivo exposure, respectively. In-vitro exposure to 0.025 or significant differences in either total formazan produced or the rate of formazan production. In-vitro exposure to 0.025mg/ml of quartz (14808607) significantly reduced both the maximum and the rate of formazan production. The author concludes that the electrooptical technique developed for this study is the first to quantify SO release from single cells and should be useful for examining single cell dynamics, heterogeneity, and the activation of the superoxide-producing system.
NIOSH-Grant; Pulmonary-system-disorders; Cell-damage; Alveolar-cells; Mineral-dusts; Dust-inhalation; Laboratory-animals
Anatomy West Virginia University Department of Anatomy Morgantown, W VA 26506
Final Grant Report
NTIS Accession No.
College of Medicine, University of Arizona, Tucson, Arizona
West Virginia University, Morgantown, West Virginia