Chemiluminescence from macrophages and monocytes.
Castranova-V; Lee-P; Ma-JY; Weber-KC; Pailes-WH; Miles-PR
Cellular Chemiluminescence 1987; :3-19
The characteristics of chemiluminescence in alveolar macrophages, peritoneal macrophages, and blood monocytes were reviewed with regard to the monitoring of the phagocytic process. The production of chemiluminescence was associated with an increase in oxygen consumption and the release of reactive forms of oxygen including the superoxide anion and hydrogen-peroxide. Topics discussed included the methods for the measurement of chemiluminescence from peritoneal macrophages, alveolar macrophages, and blood monocytes; sources of chemiluminescence in alveolar macrophages and the role of reactive forms of oxygen in zymosan stimulated chemiluminescence; the effect of luminol in enhancing the amount of chemiluminescence generated from alveolar macrophages; the relationship between species, response, and stimulant with regard to the differential requirement for opsinization for the particulate induced activation of chemiluminescence from alveolar macrophages; the enhancement of alveolar macrophage chemiluminescence by in-vitro exposure to surfactant; and the adverse effects of bronchodilators on chemiluminescence derived from alveolar macrophages.
Cell-metabolism; Blood-cells; Pulmonary-system; Enzymatic-effects; Oxidative-processes; Airborne-particles; Phagocytic-activity
Asthma and Chronic Obstructive Pulmonary Disease; Disease and Injury; Pulmonary-system;