Comparative Antimutagenicity of Chlorophyllin and Five Other Agents against Aflatoxin B1-Induced Reversion in Salmonella typhimurium Strain TA98.
Whong-Z; Stewart-J; Brockman-HE; Ong-T
Teratog, Carcinog, Mutagen 1988 Jan; 8(4):215-224
The antimutagenic activity of chlorophyllin against aflatoxin-B1 (1162658) was compared to that of vitamin-A, vitamin-C, vitamin-E, retinoic-acid, and beta-carotene using the plate incorporation reversion test in the presence of S9 activation with the Salmonella- typhimurium strain (TA-98). Aflatoxin-B1 induced reversion was inhibited by chlorophyllin, vitamin-A, retinoic-acid, and beta- carotene with maximal inhibition of 100, 100, 55, and 45 percent, respectively. Chlorophyllin induced inhibition of aflatoxin induced reversion occurred only when the agent was administered at the time of the aflatoxin treatment. The retinoids, vitamin-A and retinoic- acid, produced the greatest inhibition of aflatoxin mutagenicity at a plate concentration of 26 nanomoles; but the rank order of inhibitory activity from highest to lowest at plate concentrations of 860 and 2600 nanomoles was chlorophyllin, vitamin-A, retinoic- acid, and beta-carotene. The agents tested showed no lethal effects against the S-typhimurium strain (TA-98), and no toxic effects were observed against the background lawn growth on the plates used to count the revertant colonies. None of the compounds selected against the revertants of (TA-98).
NIOSH-Author; Mutagens; Mycotoxins; Bacterial-cultures; Microbial-test-systems; Vitamins; Mutagenesis;
Author Keywords: vitamin A; vitamin C; vitamin E; retinoic acid; B-carotene
Wen-Zong Whong, NIOSH, 944 Chestnut Ridge Road, Morgantown, WV 26505
Teratogenesis, Carcinogenesis, and Mutagenesis