Mass balance and dose accountability in percutaneous absorption studies: development of non-occlusive application system.
Bucks-DA; Maibach-HI; Guy-RH
Departments of Pharmaceutical Chemistry and Dermatology, Schools of Pharmacy and Medicine, University of California, San Francisco, California, 1987 Dec; :1-9
A new in-vivo method was developed to account for topically applied doses in the study of percutaneous absorption. A protective, but not occlusive, covering device was used which allowed free passage of passive transepidermal water loss, retained exfoliated corneocytes on which a fraction of the applied compound may have been sequestered, and constrained the administered compound to the application site. The semipermeable membrane Gore-tex was used in the device; it provided a membrane that had a pore size of 0.2 microns which was much smaller than the dimensions of a corneocyte and a negligible barrier to the escape of transepidermal water loss from beneath the device. The patch system was applied to the ventral forearms of ten normal healthy volunteers. Transepidermal water loss was measured above the chambers on the skin, below chambers, and on skin adjacent to the chambers at 24 hours post application. The occlusivity of the unmodified chambers and the ability of the protective devices to allow transepidermal water loss was determined. This device, while used thus far only in human testing, should be useful in dermal exposure studies on test animals, according to the authors.
NIOSH-Grant; Dermatitis; Skin-exposure; Laboratory-animals; Skin-tests
Pharmacy University of California 926 Medical Sciences Building San Francisco, Calif 94143
Final Grant Report
NTIS Accession No.
Departments of Pharmaceutical Chemistry and Dermatology, Schools of Pharmacy and Medicine, University of California, San Francisco, California
University of California San Francisco, San Francisco, California