A carcinogen/mutagen detector for HPLC.
Department of Radiology, Case Western Reserve University, Cleveland, Ohio 1987 Dec; :1-10
A project to develop a detector to measure chemical carcinogens was carried out. This new detector was to be sensitive enough to be compatible with results obtained using high performance liquid chromatography (HPLC). The basis for the detector was the difference in the rates at which carcinogens and noncarcinogens attach excess electrons. This difference was discovered in pulse conductivity studies of the attachment rate constants (k(e)) of excess electrons. A rigorous validation study of the k(e) test was completed and reported. The k(e) test results for 152 chemicals which had been previously screened were compared with newly published data on the carcinogenic properties of chemicals. The accuracy of the k(e) test was superior to that of the Ames bioassay. The use of the k(e) test in a carcinogen screening battery and the cost effectiveness of the k(e) test were included. In occupational studies, normal phase HPLC was demonstrated as an efficacious means of separating a class of chemical carcinogens frequently found at the workplace such as polycyclic aromatic hydrocarbons. Samples of coal-tar (8007452) and diesel emissions were separated using normal phase HPLC which illustrated that such samples could be screened with the carcinogen detector. Extracts from coal-tar-oil (8001589) and diesel emission particulates were screened with the k(e) and Ames tests and preliminary results indicated that the k(e) test could be used in this application.
NIOSH-Grant; Cancer; Carcinogens; Mutagens; Bioassays; Diesel-exhausts; Coal-workers; Coal-products; Analytical-methods
Radiology Case Western Reserve Univ 2065 Adelbert Road Cleveland, Ohio 44106
Final Grant Report
NTIS Accession No.
Department of Radiology, Case Western Reserve University, Cleveland, Ohio
Case Western Reserve University, Cleveland, Ohio