A study of allergic contact dermatitis caused by chrysanthemums -- characterization of allergens by skin tests on growers and sensitized guinea pigs.
Bando-A; Imura-T; Kimura-M; Murata-T; Bando-S; Kubo-H; Suzuki-H; Niki-Y; Fujita-T
Proceedings of the VII International Congress of Rural Medicine, Salt Lake City, Utah, September 17-21, International Association of Agricultural Medicine 1978 Sep:77-80
Growers of ornamental chrysanthemums in the Tokushima Prefecture of Japan have suffered from contact dermatitis caused by this plant. Patch tests were carried out using the leaves of chrysanthemums, crude extracts of the leaves, and eight widely used agricultural chemicals. The crude extract, the leaves themselves, the leaves of cultivar kosetsu, and the chemicals gave 27.3, 20.5, 7.3, and 3.6 percent positive responses, respectively, suggesting that the allergen was present in the leaves themselves. The extract samples were taken from fresh leaves of the cultivar kinriki with water and organic solvents and separated into ten fractions. Patch tests were then made with the fractions on 141 farmers. The fractions were also tested on the skins of Hartley-guinea-pigs. Several of the fractions caused strong irritant effects. Several of the animals were injected with an emulsification of juice from the leaves in Freund's complete adjuvant in efforts to sensitize them. They were given two injections, 4 days apart, and challenged 10 days after the second injection by exposure to the irritants. These studies indicated that chrysanthemums contain two types of allergens, those which are soluble in hexane and include sesquiterpene lactones, and those which are soluble in water and include proteins.
NIOSH-Grant; Dermatitis; Allergic-dermatitis; Allergic-reactions; Farmers; Agricultural-workers; Gardeners; Laboratory-animals; Contact-allergies; Plant-substances
Prev Med & Environmental Hlth University of Iowa Inst/agric Med & Environ Hlth Iowa Oakdale, Iowa 52319
Allergic and Irritant Dermatitis; Disease and Injury; Dermatitis;
Proceedings of the VII International Congress of Rural Medicine, Salt Lake City, Utah, September 17-21, International Association of Agricultural Medicine
University of Iowa, Iowa City, Iowa