Heterogeneity in dog red blood cells: sodium and potassium transport.
J Gen Physiol 1979 Jan; 73(1):61-71
Heterogeneous characteristics of sodium ion (Na+) and potassium ion (K+) transport mechanisms in dog red blood cells (RBCs) were evaluated. Blood was drawn from adult dogs and the RBCs isolated by centrifugation. After incubation in isotonic potassium-chloride (KCl), the cells were further separated by centrifugation into subgroups with different cell volumes and different transport characteristics; red cells which swelled in isotonic KCl exhibited a higher permeability to radiolabeled K+ and possessed a greater volume dependence for transport of K+ than those red cells which shrank. A high Na+ permeability characterized cells which shrank in isotonic KCl; such cells exhibited a larger volume dependent Na+ flux than those red cells which swelled. The two subgroups of red cells did not appear to represent cell populations of different ages. The authors conclude that the population of normal RBCs in dogs is heterogeneous in that the volume dependent changes in Na+ and K+ permeability are distributed between different cell types rather than representing a single cell type which reciprocally changes its selectivity for the two ions; the basis for the volume response as well as the differential ion selectivity of cells in the two subgroups is not known.
NIOSH-Author; Transport-mechanisms; Animal-studies; Red-blood-cells; Cell-function; Kinetics; Physiological-function; Biochemical-analysis
Journal of General Physiology