Reproductive toxicity of 2,4-dinitrotoluene in the rat.
Bloch E; Gondos B; Gatz M; Varma SK; Thysen B
Toxicol Appl Pharmacol 1988 Jul; 94(3):466-472
The toxic reproductive effects of the chemosterilant 2,4- dinitrotoluene (121142) (DNT) were evaluated in the rat. Male Sprague-Dawley-rats were fed 0, 0.1, or 0.2 percent DNT for a period of 3 weeks. Blood samples were collected for assessment of luteinizing-hormone (LH), follicle-stimulating-hormone (FSH), and testosterone levels prior to sacrifice. Testes and epididymides were then removed for ultrastructural evaluation and sperm reserve counts. DNT exposure at a concentration of 0.2 percent was associated with a marked change in Sertoli cell morphology. No Leydig cell changes were detected. Alterations induced by DNT included varying sized vesicles associated with swollen mitochondria and distended endoplasmic reticulum, increased levels of circulating LH and FSH, reduced weights of epididymides, and decreased epididymal sperm reserves (63 percent reduction in animals treated with 0.2 percent DNT). Testosterone levels were unaffected by DNT. Sperm concentrations and serum hormone levels were not significantly affected by 0.1 percent DNT. The authors conclude that DNT can induce testicular injury, directly or indirectly disturb pituitary function, and exert a toxic effect at late stages of spermatogenesis. The Sertoli cell may be a locus of DNT induced inhibition of spermatogenesis and altered testicular/pituitary endocrine function.
NIOSH-Publication; NIOSH-Grant; Laboratory-animals; Endocrine-system; Reproductive-effects; In-vivo-studies; Reproductive-hazards; Toxic-effects; Toluenes; Nitro-compounds; Gonadotropic-hormones; Cell-damage
Laboratory Medicine Albert Einstein Coll of Med 1300 Morris Park Avenue Bronx, N Y 10461
Toxicology and Applied Pharmacology
Yeshiva University, New York, New York