Pathomechanisms of chemically induced depigmentation.
Supplemental Information 1983 Jul; :1-7
High pressure liquid chromatographic analyses were made of skin from albino-mice and pigmented-mice, and melanoma cells (B16 transplantable hamster melanoma). Under the conditions of system-A, which detects dopa, cysteinyldopas and related compounds, the melanoma cells demonstrated six peaks. Pigmented ear skin showed four of these peaks and albino ear skin showed only one. Using system-B, which detects indole derivatives, two peaks were observed. Enzyme studies demonstrated that three glutathione metabolizing enzymes were elevated after exposure to 4-tertiary-butyl-catechol (98293) (TBC), an antioxidant. The food additive butylated- hydroxytoluene (128370) (BHT) was added to the food given to C57BL/6N-mice. Four weeks after feeding BHT the pigment was sparse and irregular. Electron microscopy studies were carried out on human skin from patients before and after treatment with PUVA. After 2 weeks of treatment many melanosomes demonstrated irregular deposition of pigment which was ultrastructurally interpreted as the start of pheomelanogenesis. Skin biopsy of a black woman with depigmentation who wore rubber gloves while working at a hotel showed no melanocytes. Tests revealed melanosomes with a pheomelanogenesis like ultrastructure. The author concludes that the basic methods needed to investigate chemically induced depigmentation have been developed.
NIOSH-Grant; Chromatographic-analysis; Laboratory-animals; Skin-exposure; Pigmentation-disorders; Skin-disorders
Dermatology University of California Department of Dermatology San Francisco, CA 94143
Final Grant Report
NTIS Accession No.
University of California San Francisco, San Francisco, California