Effects of 4-tertiary butyl catechol on glutathione-metabolizing enzymes in vivo and in vitro.
Kawashima T; Yonemoto K; Gellin GA; Epstein WL; Fukuyama K
Department of Dermatology, University of California, San Francisco, California, 1988 Jan; :1-28
The effects of noncytotoxic doses of 4-tertiary-butyl-catechol (98293) (TBC) on glutathione-S-transferase (GST) activity in the skin of male, 2 month old Uscd-mice and B16-murine-melanoma cells in culture were investigated. These effects were studied in relation to changes in the activities of glutathione-reductase (GR) and gamma- glutamyl-transpeptidase (GGT) which are involved in pheomelanogenesis. Electron microscopic findings in the in-vivo studies demonstrated an altered form of melanosomes, classified as pheomelanosomes, in the TBC treated animals. Ear tissues demonstrated increased activities for all enzymes tested following TBC application. These increases were 27, 19, and 35 percent for GR, GST, and GGT, respectively. In-vitro studies demonstrated a dose dependent decrease in the eumelanin content. GR activity in TBC treated cells demonstrated a 12 percent elevation already at 12 hours. This continued to be higher than controls throughout the study period. No significant difference in GGT was noted at 12 hours, but it began to elevate at 24 hours, reaching a 26 percent increase after 48 hours. GST also did not increase during the first 12 hours, but showed a 25 percent increase by 48 hours after exposure. The authors suggest that these findings may aid in the construction of an experimental model to investigate the molecular action of other more commonly used chemical depigmenting agents.
NIOSH-Grant; Pigmentation-disorders; Antioxidants; Laboratory-animals; Catechols; In-vivo-studies; In-vitro-studies; Enzyme-activity
Dermatology University of California Department of Dermatology San Francisco, Calif 94143
Final Grant Report
NTIS Accession No.
Department of Dermatology, University of California, San Francisco, California
University of California San Francisco, San Francisco, California