Pathogenesis of Allergic Pulmonary Aspergillosis.
School of Medicine, St. Louis University, St. Louis, Missouri, Terminal Progress Report :21 pages
Efforts were made to establish an animal model and advance the understanding of the epidemiology of allergic aspergillosis. Immunized and unimmunized monkeys with and without immunoglobulin (Ig) G precipitating antibody to Aspergillus-fumigatus (AF) were injected at multiple sites intradermally with normal human serum or serum high in IgE against AF. Each site was injected with AF 1 day later. Profound skin changes were associated with simian antibody (IgG) and human antibody (IgE) directed against AF. Changes consisted of perivascular and interstitial neutrophilic infiltration at 2 hours, eosinophils at 6, and mononuclear cells at 24 hours. To develop the primate model of allergic aspergillosis, monkey-A received no immunization and had no precipitating antibody to A- fumigatus. Monkey-B was immunized with A-fumigatus and developed strong precipitating antibody in his serum for A-fumigatus. On the first day monkey-A received normal human serum and monkey-B received human serum with high titer of IgE antiaspergillus antibody. On concentration of bronchial lavage fluid, both monkeys demonstrated IgA, IgG, and IgM, but only monkey-B demonstrated antibody activity directed toward A-fumigatus. Samples taken of the urban atmosphere in St. Louis indicated the A-fumigatus spore counts in St. Louis were comparable to those obtained in similar studies in the United Kingdom. The lower reported incidence of this disease in the United States as compared to the United Kingdom may result from a failure to recognize this disease entity.
NIOSH-Grant; Laboratory-animals; Lung-disorders; Fungi; Fungal-diseases; Immunoglobulins; Molds; Allergic-disorders;
Internal Medicine St Louis University 1402 S Grand Blvd St Louis, MO 63104
NTIS Accession No.
School of Medicine, St. Louis University, St. Louis, Missouri, Terminal Progress Report
St. Louis University, Saint Louis, Missouri