This study was performed to determine the different reactions of various fish to carcinogenic compounds, to demonstrate the wide variety of neoplasms which can develop in fish, to demonstrate that a combination of halomethanes can induce neoplasia in fish, and to show the effect of the genotoxic compound methylazoxymethanol- acetate (592621) (MAM-Ac) in a model. Fish used in the study included the Japanese-medaka, king-cobra-guppy, sheepshead-minnow, Gulf-killifish, inland-silverside, rivulus, and fathead-minnow. The chemicals used in the study included a mixture of six trihalomethanes (THM), specifically chloroform (67663), bromodichloromethane (75274), chlorodibromomethane (124481), bromoform (75252), trichloroethylene (79016), and carbon- tetrachloride (56235). Also included were the following single compounds: vinylidene-chloride (75354), trichloroethylene, and bis(2-chloroethyl)ether (111444). The authors conclude that some small aquarium fish are good carcinogenicity models as they are sensitive to induction of a wide variety of neoplasms which have a short latency period. The fish also have a low number of spontaneous neoplasms, are cheap to maintain, and most susceptible tissues can be screened for neoplasms in one or a few microscopic slides.
Proceedings of the Fourth NCI/EPA/NIOSH Collaborative Workshop: Progress on Joint Environmental and Occupational Cancer Studies, April 22-23, 1986, Rockville, Maryland, NIH Publication No. 88-2960