A sampling and analytical method was developed to monitor worker exposure to zeranol (55331298), a nonsteroidal anabolic agent used for increasing the rate of weight gain and improving feed conversion in cattle and sheep, and also its possible metabolites, precursors, or coproducts. The collection strategy included air, area wipe, hand/dermal wipe, and work garment sampling. Both ultraviolet and fluorescence spectra were produced from standard samples of zeranol and four related compounds (taleranol, zearalanone, zearalenone, and zearanenol) in methanol. The samples were analyzed by high performance liquid chromatography. A test for extraction efficiency for zeranol in methanol and after spiking onto separate sets of air sampling filters indicated 100 percent recovery in both cases. The limits of quantification, defined as the level at which results could be reported with a maximum of 10 percent relative standard deviation, were 0.02 and 0.15 micrograms per milliliter (microg/ml), respectively, for the ultraviolet and fluorescence detectors. The limits of detection, determined by Hubaux/Vos data analysis, were analytical method was used to assess 67 air filters, 39 area wipe samples, and 33 hand gauze pad samples, yielding maximum quantities of zeranol of 1.5, 9.5, and 410 milligrams per sample, respectively. Analysis of work garment samples yielded 16 milligrams per sample. No taleranol, zearalanone, zearalenone, or zearanenol were detected. The author concludes that the method provides the sensitivity and specificity to analyze workplace environmental samples for zeranol.