Interferon induction inhibition and mutagenic activity of nitrosated coal dust extract.
Hahon-N; Booth-JA; Stewart-JD
Environ Res 1988 Apr; 45(2):213-223
The effect of nitrosated coal dust extract with and without microsomal enzymatic activation on viral interferon induction in mammalian cell cultures was studied. Rat liver S9 fraction was used for activation. Exposure to 1 to 5 milligrams of coal dust inhibited the interferon induction by influenza virus in LLC-MK2- rhesus-monkey kidney cell monolayers by more than 60 percent. A marked and comparable reduction of interferon induction was obtained by coal dust that was either nitrosated and acidified, acidified only, or untreated. The nitrosated coal dust extract was further extracted with horse serum. The soluble chemical complexes formed with fractions of high molecular weight without bioactivation were dominant in both mutagenicity and inhibition of interferon induction. Low molecular weight fractions all inhibited interferon induction comparably and significantly with or without bioactivation, with or without a metal chelator. No mutagenic activity was manifested by these serum fractions. The authors indicate that whether impairment of interferon production by a xenobiotic agent such as nitrosated coal dust is important in gastric carcinogenesis requires further study.
NIOSH-Author; Carcinogenesis; Laboratory-animals; Coal-dust; Gastrointestinal-system-disorders; Coal-miners; In-vitro-studies