Activation of guinea pig lymphocytes and mast cells by grain-dust extract.
Alam-R; Lewis-DM; Olenchock-SA
J Allergy Clin Immunol 1988 Mar; 81(3):598-604
The production of histamine releasing factor (HRF) by guinea-pig spleen cells in the presence of grain dust extract was studied. Airborne grain dusts from spring wheat were obtained from several port grain terminals in the Duluth, Minnesota and Superior, Wisconsin areas. Aqueous extracts of the dusts were cultured with nonadherent cells obtained from immunized animals for 24 hours, and the cell free supernatants were tested for histamine releasing factor activity using lung mast cells and basophils. Control assays were performed on cells incubated with lipopolysaccharide. A dose dependent relationship was established for histamine release from lung mast cells and basophils obtained from nonimmunized animals. Dose dependency was also demonstrated for the production of histamine releasing factor from spleen cells cultured in the presence of grain dust extract. Incubation of the immunized spleen cells with lipopolysaccharide resulted in no comparable stimulation of HRF. Gel chromatography of the spleen cells culture supernatant with Sephadex-G75 superfine showed activity corresponding to histamine releasing factor eluting with fractions of 50 to 70 kilodaltons and 5 to 8 kilodaltons molecular weight. The authors conclude that grain dust contains substances that may activate lymphocytes, mast cells, and basophils.
NIOSH-Author; Grain-dusts; Laboratory-animals; In-vitro-studies; Allergic-reactions; Allergens; Immunological-tests; Lung-cells
The Journal of Allergy and Clinical Immunology