Comparison of NiSO4 and NiCl2 as sensitizers in the guinea pig.
Dorn-CR; Warner-RD; Ahmed-W
Int Arch Allergy Appl Immunol 1988 Jan; 85(3):332-336
The effectiveness of nickel-sulfate (7786814) and nickel-chloride (7718549) as skin sensitizers in guinea-pigs was compared, in order to determine which compound was the most appropriate for the guinea-pig maximization test (GPMT). The GPMT was conducted in female Hartley-guinea-pigs using 0, 0.03125, 0.0625, 0.125, 0.25, or 0.5 percent nickel-chloride or nickel-sulfate intradermally or topically as sensitizing doses. Intradermal challenge with the same doses was performed 23 days later, and erythematous reactions were measured. The lymphocyte transformation test (LTT) was conducted in cells from sensitized guinea-pigs using 0.108x10(-6), 2.70x10(-6), or 5.40x10(-6) molar nickel-sulfate or nickel-chloride for comparison purposes. In the GPMT, erythematous responses at the challenge site for 0.125, 0.25, or 0.5 percent nickel-sulfate were greater than for controls when read at 24 and 48 hours. In guinea-pigs treated with nickel-chloride, the response was significantly different from controls only for 0.5 percent nickel-chloride. In the LTT, responses to both nickel-sulfate and nickel-chloride were significantly greater than that of controls. The response to nickel-sulfate was greater than that of nickel-chloride; however, the difference was not significant. The authors conclude that using nickel-sulfate as a sensitizer in the GPMT is preferable to using nickel-chloride, and that skin testing may be more valuable than the LTT for the GPMT with these sensitizers.
NIOSH-Publication; NIOSH-Grant; In-vivo-studies; Laboratory-animals; Skin-tests; Sensitization; Blood-cells; In-vitro-studies; Physiological-response; Dermatitis; Nickel-compounds
Veterinary Preventive Medicine Ohio State University 1900 Coffey Road Columbus, Ohio 43210
International Archives of Allergy and Applied Immunology
Ohio State University, Columbus, Ohio