Asbestos-Induced Mesothelioma and Chromosomal Abnormalities in Human Mesothelial Cells In Vitro.
Linnainmaa-K; Gerwin-B; Pelin-K; Jantunen-K; Lechner-JF; Harris-CC
The induction of chromosomal aberrations in human pleural mesothelial cells by asbestos (1332214) was studied in-vitro. Human primary pleural mesothelial cells obtained from noncancerous donors by pleural effusion were cultured and treated with 0 to 6.67 micrograms per square centimeter (microg/cm2) amosite (12172735) asbestos. Primary human fibroblasts were treated with 0 to 6.67microg/cm2 amosite for comparison. Cultures were examined for chromosomal aberrations and aneuploidy 24 hours after treatment. In passage experiments, primary mesothelial cells were treated twice with 0 or 0.27microg/cm2 amosite or glass fibers (14808607) during the first passage. After treatment, cultures were maintained as long as possible under close observation to detect any alterations. They were examined for chromosomal aberrations at every passage 3 to 5 days after subculturing. Amosite at 0.14 and 0.27microg/cm2 induced a significant incidence of chromosome aberrations, mostly chromatid breaks and gaps, in mesothelial cells. Doses of 0.07 and aneuploidy. In human fibroblasts, effective doses for inducing chromosome aberrations were 2.67 and 6.67microg/cm2. In passage experiments, amosite and glass fibers induced chromosome aberrations. Amosite treated cells continued to divide for several cell cycles after control cultures had senesced. Control cultures, as they senesced, accumulated large numbers of abnormal and aneuploid cells. Amosite treated mesothelial cells showed varying morphological changes. For example, cells became more fibroblastic in appearance during the fourth passage in one experiment. Altered cells had a modal chromosome number of 45 and lacked the Y- chromosome. The authors conclude that amosite and glass fibers induce chromosome aberrations in human pleural mesothelial cells. The effect is probably due to a physical property of fibers rather than a chemical component of asbestos.
In-vitro-studies; Cell-culture-techniques; Humans; Asbestos-fibers; Fibrous-glass; Dose-response; Chromosome-disorders; Cell-alteration; Chromosome-damage;
1332-21-4; 12172-73-5; 14808-60-7;
The Changing Nature of Work and Workforce, Proceedings of the Third Joint US-Finnish Science Symposium, Frankfort, Kentucky, October 22-24, 1986, NIOSH, Cincinnati, Ohio