NIOSHTIC-2 Publications Search
Covalent binding of N-hydroxy-N-acetyl-2-aminofluorene and N-hydroxy-N-glycolyl-2-aminofluorene to rat hepatocyte DNA: in vitro and cell-suspension studies.
Corbett MD; Lim LO; Corbett BR; Johnston JJ; Wiebkin P
Chem Res Toxicol 1988 Jan; 1(1):41-46
The covalent binding of N-hydroxy-N-acetyl-2-aminofluorene (53952) (NOHAAF) and N-hydroxy-N-glycolyl-2-aminofluorene (NOHGAF) to rat hepatocyte DNAwas studied in-vitro. Hepatocyte suspensions prepared from male Sprague-Dawley-rats were incubated with carbon-14 labeled NOHAAF or NOHGAF for 2 hours. The extent of binding of NOHAAF and NOHGAF to cellular DNA and RNA was determined. Both NOHAAF and NOHGAF were bound to DNA and RNA to about the same extent. Cytosolic and microsomal fractions were prepared from livers of Sprague-Dawley-rats. These were incubated with NOHAAF or NOHGAF in the presence or absence of 3'-phospho-adenosine-5'-phosphosulfate (PAPS). The rate and extent of metabolism were determined. Calf thymus DNA was incubated with NOHAAF or NOHGAF in the presence or absence of rat liver cytosol, microsomes or PAPS and the extent of NOHAAF or NOHGAF DNA binding was determined. In some experiments metabolized by rat liver cytosol. NOHAAF was metabolized at a much faster rate and to a greater extent than NOHGAF; however, this difference was largely eliminated when PAPS was present. The microsomal fraction also metabolized both substrates, NOHGAF being metabolized at a much faster rate. Binding of NOHAAF to calf thymus DNA was catalyzed by rat liver cytosol. NOHGAF binding to calf thymus DNA was also catalyzed by rat liver cytosol but only in the presence of PAPS. The microsomal preparation catalyzed binding of NOHAAF and NOHGAF to calf thymus DNA. Paraoxon inhibited NOHAAF and NOHGAF DNA binding in all cases. The authors suggest that nucleic acid adducts of NOHGAF retain a larger proportion of their acyl groups than those of NOHAAF.
NIOSH-Publication; NIOSH-Grant; Cancer; Organo-nitrogen-compounds; Amino-compounds; In-vitro-studies; Liver-cells; Laboratory-animals; Nucleic-acids; Biotransformation; Chemical-structure; Bioactivation
Michael D. Corbett, Building 120, University of Florida, Gainesville, FL 32611
Issue of Publication
Chemical Research in Toxicology
University of Florida, Gainesville, Florida
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division