Reactive forms of oxygen and chemiluminescence in phagocytizing rabbit alveolar macrophages.
Miles-PR; Castranova-V; Lee-P
Am J Physiol Cell Physiol 1978; 235(3):C103-C108
The chemiluminescent response of rabbit alveolar macrophages to foreign particles was investigated with regard to determination of the involvement of reactive forms of oxygen. The alveolar macrophages were obtained by tracheal lavage performed on New- Zealand-white-rabbits. The foreign particle used to initiate the chemiluminescent response was zymosan at a concentration of two milligrams per 10(7) macrophages. Chemiluminescence was determined in dark adapted cells as counts per minute in the tritium channel of a liquid scintillation counter operated in the out of coincidence mode. Involvement of the superoxide anion in the chemiluminescent process was assessed by including superoxide-dismutase in the reaction preparation. Inclusion of 0.4mg/ml superoxide-dismutase completely abolished the chemiluminescence of the alveolar macrophages in response to zymosan. Heat inactivated superoxide- dismutase or bovine serum albumin at the same concentration had no effect. The chemiluminescent response was also abolished by catalase and the hydroxyl radical scavengers, benzoate and ethanol. The authors conclude that the mechanism of chemiluminescence involves the extracellular reaction of zymosan with various reactive species of oxygen.
NIOSH-Author; Oxidative-processes; Physiological-chemistry; Toxic-effects; Laboratory-animals; Lung-cells; Immune-reaction; Cell-metabolism; In-vitro-studies; Phagocytes;
Author Keywords: antibacterial substances; superoxide anion
P. R. Miles, Appalachian Laboratory for Occupational Respiratory Diseases, National Institute for Occupational Safety and Health, U.S. Public Health Service, Department of Health, Educatwn, and Welfare, Morgantown, West Virginia 26505
American Journal of Physiology: Cell Physiology