Pulmonary ferruginous bodies. Development in response to filamentous dusts and a method of isolation and concentration.
Gross-P; deTreville-RT; Cralley-LJ; Davis-JM
Arch Pathol 1968 May; 85:539-546
The development and isolation of ferruginous bodies in hamster lungs were described, and a method for use with animal or human lungs was presented. Hamsters were injected intratracheally with 3.5 milligrams of fibers in aqueous suspensions. Fibers tested included ceramic aluminum-silicate (1302767) fibers with a median diameter of 2 microns, silicon-carbide (409212) whiskers with a diameter range of 0.5 to 3 microns, uncoated glass fibers with a median diameter of attapulgite (12174117) fibers under 0.1 microns in diameter, and chrysotile (12001295) fibers of ultramicroscopic dimensions. The fibers were isolated in an aqueous or alcoholic medium after digestion of the lung tissue with sodium-hypochlorite. Paraffin sections showed the injected fibers to be confined primarily to the air spaces where they were associated with free macrophages. No ferruginous bodies were found in hamsters injected with attapulgite or silicon-carbide. Ferruginous bodies formed in response to chrysotile were small segmented structures relative to the larger unsegmented forms seen in sections from animals injected with aluminum-silicate or glass. The number of ferruginous bodies present at 6 months post injection was comparable to that at 1 month post injection. Ferruginous bodies were detectable in lung smears derived from tissue digests of all treated animals except for those injected with attapulgite. Asbestos bodies from lung tissue of an asbestos worker were described in detail. The authors conclude that the similarities between the ferruginous bodies derived from chrysotile and those derived from the other filaments casts doubt on morphological distinctions between asbestos bodies and pseudoasbestos bodies.
NIOSH-Author; Respiratory-irritants; Laboratory-animals; Humans; Pulmonary-system; Mineral-dusts; Sample-preparation; Dust-particles; Fibrous-bodies; Occupational-hazards; Physiological-response; Microscopic-analysis
1302-76-7; 409-21-2; 14807-96-6; 12174-11-7; 12001-29-5
Archives of Pathology