Use of arylazido aminopropionyl ATP, a new ATP antagonist, to identify the role of purines in neuronal transmission.
Vascular neuroeffector mechanisms: 4th international symposium. Bevan JA, ed. New York: Raven Press, 1983 Jan; :165-169
A new adenosine-triphosphate (ATP) antagonist, 3'-O-(3(N-(4-azido-2- nitrophenyl)amino)propionyl)-adenosine-5'-triphosphate (ANAPP3), was used to study the role of purines in neuronal transmission in three neuroeffector systems: guinea-pig vas deferens, bladder, and taenia coli. In guinea-pig vas deferens, ANAPP3 alone at 10(-4) molar and in combination with prazosin (10(-6) molar) produced an antagonism of responses to ATP. In reserpine pretreated animals, the effect was greater, as well as in animals treated with the combination of ANAPP3 and prazosin, compared to ANAPP3 alone. Qualitative changes were also observed, such as reduced initial and secondary contractions, when the agents were combined. Phasic contractions were reduced when only ANAPP3 was used, suggesting that both norepinephrine and a purine contribute to the neurogenic response in the vas deferens. In the guinea-pig bladder, ANAPP3 exhibited an antagonistic effect on the response to ATP. When a combination of atropine and ANAPP3 was used, a depressed response was observed, suggesting that both ATP and acetylcholine work as motor transmitters. In the guinea-pig taenia coli, responses to purines were antagonized by ANAPP3; however, transmural inhibitory stimulation was not affected. The authors conclude that ATP is not the transmitter of the noradrenergic, noncholinergic nerves in taenia coli.