Toxicity of the mycotoxin patulin for rat alveolar macrophages.
Sorenson-WG; Simpson-J; Castranova-V
Environ Res 1985 Jan; 38(2):407-416
The toxicity of patulin (149291), a polyketide lactone mycotoxin, was studied in alveolar macrophages (AM) harvested from male Long- Evans-hooded-rats. Exposure to patulin for a 2 hour period caused a significant increase in mean cell volume (MCV) whereas there was no increase in MCV after a 1 hour exposure interval. The presence or absence of glucose had no effect on MCV. Both time and concentration dependent relationships were noted for the release of chromium from AM following exposure to patulin. Significant leakage of chromium was noted within 30 minutes of treatment with concentrations greater than 0.15 millimolar (mM) patulin. Marked inhibitions of ATP concentrations in AM monolayer cultures were noted within 1 hour following exposures to 0.05mM patulin. Patulin also inhibited the incorporation of tritium labeled precursors into protein and RNA. The authors indicate that this inhibition of critical cellular functions in cultured alveolar macrophages suggests that inhalation of airborne silage or grain dust particulates contaminated with patulin could have harmful effects on normal macrophage functions in workers.
NIOSH-Author; Agricultural-workers; Farmers; Grain-elevator-workers; Mycotoxins; Airborne-particles; Alveolar-cells; Cell-cultures