Prevention of asbestos-induced cell death in rat lung fibroblasts and alveolar macrophages by scavengers of active oxygen species.
Shatos MA; Doherty JM; Marsh JP; Mossman BT
Environ Res 1987 Oct; 44(1):103-116
The possible protective effects of antioxidants against the cytotoxic effects of asbestos was evaluated in lung fibroblasts and alveolar macrophages of rats. A diploid line of rat lung fibroblasts was used. Alveolar macrophages were obtained by bronchoalveolar lavage. The antioxidants, superoxide-dismutase, catalase, dimethylthiourea, and deferoxamine were incubated in various concentrations with the cells in the presence of 15 micrograms of crocidolite (12001284) asbestos for 24 hours. The viability of the cells was assessed by the trypan-blue or nigrosin dye exclusion tests. In the absence of antioxidants, the viability of macrophages was 63.4 percent after exposure to 25 micrograms per square centimeter (microg/cm2) of crocidolite, and that of fibroblasts was 48.3 percent after exposure to only 15microg/cm2. The presence of any one of the antioxidants caused a dose dependent reduction of the cytotoxicity. The authors suggest that asbestos may induce the formation of active oxygen species in cells of the lung which then are responsible for the cytotoxic effects. They conclude that the protective effects of the antioxidants are due to their oxygen scavenging abilities.
NIOSH-Publication; NIOSH-Grant; Asbestos-fibers; Lung-cells; Cell-damage; Cell-cultures; Cytotoxic-effects; Antioxidation; Oxidation-reduction-reactions
Pathology University of Vermont Burlington, VT 05405
University of Vermont & St Agric College, Burlington, Vermont