Airway epithelium modulates the reactivity of guinea-pig respiratory smooth muscle.
Hay-DW; Farmer-SG; Raeburn-D; Robinson-VA; Fleming-WW; Fedan-JS
Eur J Pharmacol 1986 Sep; 129(1-2):11-18
The reactivity of guinea-pig isolated trachea was studied with and without removal of airway epithelium. Dose response contractile curves were obtained for potassium-chloride (KCl), histamine, and methacholine in the presence and absence of indomethacin. Epithelium removal had no significant effect on the mechanical properties of isolated tracheal strips but increased the sensitivity of the tissue to both histamine and methacholine. The maximum contractile response of the tissue after epithelium removal was increased for histamine but was unchanged for methacholine. Epithelium removal yielded variable results for maximum contractile response to KCl treatment. Indomethacin incubation induced tissue relaxation in intact and epithelium denuded tracheal strips. Indomethacin treatment enhanced the maximal contractile response to KCl in the absence of epithelium and increased tissue sensitivity to methacholine in intact preparations. Indomethacin had no effect on tissue responses to histamine. The authors conclude that inhibitory and excitatory factors from epithelial cells regulate the reactivity of guinea-pig isolated airway smooth muscle.
NIOSH-Author; Laboratory-animals; Lung-tissue; In-vitro-studies; Physiological-response; Neuromuscular-function; Pulmonary-function; Muscle-cells; Dose-response
European Journal of Pharmacology