Comparison of the endotoxin content of bulk cotton and cotton dust with the interleukin 1 inducing activity of cotton extracts.
Lewis-DM; Olenchock-SA; Castellan-RM
Cotton dust: proceedings of the Tenth Cotton Dust Research Conference, beltwide cotton research conferences, January 8-9, 1986, Las Vegas, Nevada. Jacobs RR, Wakelyn PJ, eds. Memphis TN: The National Cotton Council of America, 1986 Apr; :84-86
The relationship between the endotoxin content of extracts of cotton samples and cotton dust and the interleukin-1 (IL1) inducing activity and ability to induce pulmonary damage in human subjects was studied. Alveolar macrophages from rats were exposed to seven samples of bulk cotton and IL2 content of the cells was determined by a thymocyte proliferation assay. The endotoxin content of the bulk samples and airborne dust generated when the samples were carded was measured using the quantitative chromogenic limulus amebocyte lysate assay. The acute pulmonary response of healthy human subjects exposed to the dust in the Clemson model card room was also determined by measuring forced expiratory volume in 1 second (FEV1). The correlation coefficient between endotoxin content and IL1 inducing activity was 0.78, significant at the 0.01 level. The endotoxin content of the airborne dust and bulk cotton did not correlate well. However, airborne endotoxin levels correlated well with the decline in FEV1, which was proportional to the increase in endotoxin level. The authors conclude that acute pulmonary reaction to cotton dust correlates well with levels of endotoxin in airborne dust.
Airborne-dusts; Cotton-dust; Pulmonary-function-tests; Respiratory-irritants; Pulmonary-system-disorders; Endotoxins; Cell-cultures; Humans; Lung-cells
Cotton dust: proceedings of the Tenth Cotton Dust Research Conference, beltwide cotton research conferences, January 8-9, 1986, Las Vegas, Nevada