Application of SOS umu-test for the detection of genotoxic volatile chemicals and air pollutants.
Ong-T; Stewart-J; Wen-Y; Whong-Z
Environ Mutagen 1987 Apr; 9(2):171-176
The induction of SOS function by volatile chemicals and air pollutants was studied. The testing organism was Salmonella- typhimurium (TA-1535/pSK1002). Volatile chemicals were placed in a vaporizer. Air flow through the vaporizer supplied the mutagen that impinged into a trapping medium containing bacterial tester cells. Diesel emission was generated by an idling diesel truck. Welding fumes were produced with manual arc welding. Ethylene-dibromide (106934) (EDB), propylene-oxide (75569) (PO), methyl-bromide (74839) (MB), diesel exhaust, welding fumes, and cigarette smoke showed treatment related responses with or without S9. The SOS function more than doubled compared to control. The responses were higher without S9 than with S9. EDB and cigarette smoke appeared to be weak SOS function inducers. A 2 hour long exposure to diesel emission and 6 hours to welding fumes elicited almost a tenfold increase in enzyme activities. Cigarette smoke and diesel emission were relatively toxic to the tester cells. MB and PO induced an approximate threefold increase in enzyme activities. A comparison of the results of this study with the Ames S-typhimurium assay appeared to indicate that both the SOS umu test and Ames assay have similar sensitivities to EDB and cigarette smoke. The SOS umu test was much more sensitive than the Ames assay to welding fumes, while with diesel emission SOS umu test seemed to be slightly more sensitive than the Ames assay. MB induced a significant SOS response with only 30 minute impingement. The authors conclude that the SOS umu test is potentially useful for the detection of airborne genotoxic agents.
NIOSH-Author; Toxic-materials; Volatiles; Exhaust-gases; Arc-welding; Cigarette-smoking; Bromine-compounds; Oxides; Aliphatic-compounds; In-vitro-studies
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