Development of an in situ test system for detection of mutagens in the workplace.
Ong-T; Stewart-JD; Tucker-JD; Whong-Z
Short-term bioassays in the analysis of complex environmental mixtures IV. Waters MD, Sandhu SS, Lewtas J, Claxton L, Strauss G, eds. Environmental science research, New York: Plenum Press, 1985 Aug; 32:25-36
An in-situ assay system was described in which both mutagenic airborne particles and vapors or gases may be trapped simultaneously into trapping medium containing tester cells. To be effective, an in-situ mutagenicity system must have highly sensitive indicator cells that can detect mutagens at very low concentrations and efficient systems that collect representative whole air samples and promote efficient contact with the tester cells. For most studies, bacterial cells were used. During the experiment air was drawn into the sampling device by a pump at a flow rate of 3 liters per minute. The air flow passed through a cyclone, which removed nonrespirable particles. Respirable particles and vapors or gases along with air were impinged into the trapping medium. For survival, 100, 500 and 2500 cells were plated separately onto three culture medium agar plates. For mutagenic response, samples were plated onto Ames minimal agar plates. Survivors and His+ revertants were scored after 2 days of incubation at 37 degrees-C. Heparinized human peripheral lymphocytes from healthy unrelated nonsmokers were used for the sister chromatid exchange (SCE) assay. The results from laboratory studies and the studies with side stream cigarette smoke and diesel exhaust suggested that this in-situ microbial mutagenicity test system involving impingement is potentially useful for the on site detection of airborne mutagens in the workplace. Preliminary SCE studies indicate the system may be used for cultured mammalian cells and other genetic end points.
Biological-monitoring; Air-quality-monitoring; Chromosome-damage; Cell-cultures; Mutagens; Health-hazards; Inhalants; In-vitro-studies; Microbial-test-systems
Waters-MD; Sandhu-SS; Lewtas-J; Claxton-L; Strauss-G
Short-term bioassays in the analysis of complex environmental mixtures IV