Pulmonary function analysis of bronchoconstriction: a survey of the potential etiological agents and mediators following cotton dust extract inhalation in rabbits.
Bishop-MP; Pilia-PA; Moorman-WJ; Ainsworth-SK
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana. Wakelyn PJ, Jacobs RR, eds. Memphis, TN: The National Cotton Council of America; 1985 Jan; :178-181
The effects of cotton dust and other bronchoconstrictive agents on pulmonary function were studied in rabbits. Groups of five female New-Zealand-White-rabbits underwent bronchochallenge with physiological saline followed by cotton dust extract, cotton bract extract, purified endotoxin, endotoxin depleted cotton dust extract, and N-formyl-methionyl-peptide (NFMP), and potential mediators of bronchoconstriction 5-hydroxytryptamine (50679) (5HT) and prostaglandin-F2alpha (551111) (PGF). Methacholine was used as a positive control. The effects of the agents on pulmonary function were evaluated by monitoring respiratory resistance with a computerized pulmonary mechanics analyzer for 60 minutes after challenge. The rabbits were classified as mild, moderate, or severe responders depending on whether the pulmonary resistance increased by 125 to 149, 150 to 199, or 200 or more percent after challenge, respectively. Saline caused no bronchoconstriction. NFMP caused the most consistent response, with four rabbits showing a moderate to severe response. Cotton dust and bract extracts produced moderate to severe responses in three of five animals. Only one rabbit responded, moderately, to endotoxin and two responded mildly to endotoxin depleted cotton dust. Both 5HT and PGF caused a moderate and a severe response in two rabbits. All rabbits responded to methacholine, showing a severe response within 5 minutes. The authors conclude that rabbits can be used for quantitatively measuring respiratory resistance and other pulmonary parameters following bronchoprovocation.
In-vivo-studies; Laboratory-animals; Pulmonary-function; Pulmonary-function-tests; Cotton-dust; Airway-resistance; Physiological-response; Endotoxins
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana