Stimulation of interleukin-1 production by alveolar macrophages by endotoxin from various bacterial species.
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana. Wakelyn PJ, Jacobs RR, eds. Memphis, TN: The National Cotton Council of America; 1985 Jan; :157-159
The ability of endotoxins from various bacterial species to stimulate production of interleukin-1 (IL1) from alveolar macrophages was studied in-vitro. Samples of purified lipopolysaccharide (LPS) and endotoxin from Escherichia-coli were incubated with alveolar macrophages from Lewis-rats. The cultures were assayed for IL1 using a mouse thymocyte proliferation assay. Dose response curves were constructed. The dose response curves of the LPS and Escherichia-coli endotoxin were very similar, indicating that the LPS moiety in the endotoxin molecule was responsible for the IL1 inducing activity. Purified LPS samples isolated from Enterobacter-agglomerans were also tested. The dose response curve for Enterobacter-agglomerans was shifted to the left of the one for Escherichia-coli. The concentrations of the Enterobacter- agglomerans LPS preparations required to produce half of the maximal induction of IL1 were approximately 1.4 to 8 times lower than that for the Escherichia-coli LPS. The author concludes that LPS derived from Enterobacter-agglomerans is a more potent inducer of IL1 in rat alveolar macrophages than Escherichia-coli LPS. The relative potency of the Enterobacter-agglomerans preparations varies. This may be related to the purity of the preparations.
In-vitro-studies; Microbial-test-systems; Microorganisms; Dose-response; Molecular-structure; Bioassays; Immune-reaction; Alveolar-cells
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana