Testicular toxicity following oral administration of tri-O-cresyl phosphate (TOCP) in roosters.
Somkuti SG; Lapadula DM; Chapin RE; Lamb JC IV; Abou-Donia MB
Toxicol Lett 1987 Aug; 37(3):279-290
The testicular toxicity of orally administered tri-o-cresyl- phosphate (78308) (TOCP) was studied in roosters. Adult leghorn- roosters were administered single doses of TOCP or parathion (56382), an acetylcholinesterase (AChE) inhibitor, at 750 and 5mg/kg, respectively. Two other groups of animals received 18 daily doses of TOCP or parathion at 100 and 0.1mg/kg, respectively. Enzymatic, histological, and sperm motility analyses were performed. Significant inhibition of neurotoxic-esterase (NTE) activity in the brain and testis and a slight decrease in brain AChE activity were found in the animals which had received single or multiple doses of TOCP, compared to untreated controls. Sperm motility was found to be greatly decreased in the TOCP treated roosters, and histological examination showed vacuolation of and disorganization in the seminiferous epithelium. Body weight decreases of 17 percent in the TOCP treated animals were not considered to contribute to testicular toxicity. Animals treated with single or multiple doses of parathion were found to have 100 and 24 percent decreased levels of brain AChE activity, respectively. However, they had no changes in NTE activity or testicular histology. The authors conclude that the testicular effects caused by TOCP are not caused by AChE inhibition.
NIOSH-Publication; NIOSH-Grant; Organo-phosphorous-compounds; Testes; Spermatozoa; Neurotoxins; Laboratory-animals; In-vivo-studies; Enzyme-activity; Enzyme-inhibitors;
Author Keywords: Organophosphorus compound; delayed neurotoxicity; neurotoxic esterase activity
Pharmacology Duke University Department of Pharmacology Durham, N C 27710
Duke University, Durham, North Carolina