Mutagenicity of diesel exhaust particles and oil shale particles dispersed in lecithin surfactant.
Wallace-WE; Keane-MJ; Hill-CA; Xu-J; Ong-T
J Toxicol Environ Health 1987 May; 21(1-2):163-171
The mutagenic activity of pulmonary surfactant (dipalmitoyl- lecithin) dispersed diesel exhaust particulate material from exhaust pipe scrapings, diluted automobile diesel exhaust particulate material collected on filters, and two oil shale ores, were compared to 0.85 percent physiological saline dispersed samples and to dichloromethane extracted samples. Mutagenicity testing was performed by the Ames Salmonella/microsome assay. Responses of the dichloromethane extracted and the dipalmitoyl-lecithin dispersed samples were greater than those observed with saline dispersed samples, although mutagenicity increased with dose in all cases. Addition of S-9 fraction reduced the mutagenicity of all samples, but particularly the dipalmitoyl-lecithin dispersed one. Dispersed rather than dissolved soot fractions were shown to be responsible for mutagenic activity. These results indicate that mutagenic agents (diesel soots) are mutagenic in the Ames Salmonella assay when dispersed in a phospholipid emulsion resembling pulmonary surfactant material. The authors conclude that mutagens associated with inhaled particles may be similarly dispersed into the phospholipid component of pulmonary surfactant in-vivo and effect cellular mutagenesis.
NIOSH-Author; Lung-lesions; Lung-tissue; Solvent-extraction; Oil-shale; Toxic-effects; Polycyclic-aromatic-hydrocarbons; Gene-mutation; Particulates; Aqueous-solutions; Solvents; Diesel-exhausts; In-vitro-studies
Journal of Toxicology and Environmental Health