The distribution and nature of chromium (7440473) binding sites were studied in-vitro. Chinese-hamster ovary cell cultures were incubated with chromium-51 (Cr-51) labeled sodium-chromate (7775113) at a concentration of 60 nanomolar for 2 or 24 hours. The cells were washed, homogenized, and separated into fractions by centrifugation. The Cr-51 activity and protein content of each fraction were measured. The supernatant from the centrifuged homogenate was dialyzed and examined by anion exchange and ion pair high performance liquid chromatography. At least 75 percent of the Cr-51 activity was found in the nonsedimentable cytosolic fraction. A small, significant amount of radiolabel was found in the nuclear fraction. Dialysis through a membrane with a 12000 dalton cutoff caused a loss of 90 percent of the Cr-51 and 60 percent of the protein after 18 hours. Chromatographic analyses indicated the presence of at least three different molecular species interacting with chromate or its reduced derivative trivalent chromium (16065831). Two species were identified as glutathione and nucleoside diphosphates and triphosphates. The third species appeared to be a protein or peptide, eluting under the same conditions as metallothionein. The glutathione accounted for 50 percent of the observed Cr-51 activity and the nucleotides 30 percent. The remaining activity was due to the unknown species. The authors note that most of the chromate taken up by nucleated cells is not protein bound but resides in small, easily dialyzable molecules.
Links with this icon indicate that you are leaving the CDC website.
The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website.
Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website.
You will be subject to the destination website's privacy policy when you follow the link.
CDC is not responsible for Section 508 compliance (accessibility) on other federal or private website.
For more information on CDC's web notification policies, see Website Disclaimers.
CDC.gov Privacy Settings
We take your privacy seriously. You can review and change the way we collect information below.
These cookies allow us to count visits and traffic sources so we can measure and improve the performance of our site. They help us to know which pages are the most and least popular and see how visitors move around the site. All information these cookies collect is aggregated and therefore anonymous. If you do not allow these cookies we will not know when you have visited our site, and will not be able to monitor its performance.
Cookies used to make website functionality more relevant to you. These cookies perform functions like remembering presentation options or choices and, in some cases, delivery of web content that based on self-identified area of interests.
Cookies used to track the effectiveness of CDC public health campaigns through clickthrough data.
Cookies used to enable you to share pages and content that you find interesting on CDC.gov through third party social networking and other websites. These cookies may also be used for advertising purposes by these third parties.
Thank you for taking the time to confirm your preferences. If you need to go back and make any changes, you can always do so by going to our Privacy Policy page.