Human erythrocyte glutathione s-transferase: A possible marker of chemical exposure.
Ansari-GA; Singh-SV; Gan-JC; Awasthi-YC
Toxicol Lett 1987 Jun; 37(1):57-62
The possible use of the degree of inhibition of glutathione-S- transferase (GST) activity as a biological marker for determining exposure to chemicals such as acrolein (107028), styrene-oxide (96093), propylene-oxide (75569), ethylene-dibromide (106934), and ethylene-dichloride (107062) was explored. GST activity was studied in-vitro in human erythrocytes or as the purified enzyme. While GST activity was inhibited by all these compounds, acrolein was the most inhibitory. A dose dependent inhibition was evident in each case not only for inactivation of erythrocyte GST in-situ but for inhibition of purified erythrocyte GST as well. Concentrations inhibiting 50 percent of the activity (I50) ranged from around 10(- 3) to 10(-4) molar. Some of the I50 values for the compounds used in this study were relatively high. The authors state that the concentrations of these chemicals in the blood of chronically exposed industrial workers may not reach these levels. It is suggested that further studies be made to evaluate the usefulness of inhibition of erythrocyte GST by these agents. The authors conclude that chemical exposure will result in the reduced capacity of erythrocyte GST to detoxify the xenobiotics.
NIOSH-Publication; NIOSH-Grant; Enzyme-activity; Epoxides; Humans; In-vitro-studies; Red-blood-cells; Dose-response; Screening-methods; Biological-monitoring; Ethylenes; Toxic-materials;
Author Keywords: Biological marker; chemical exposure; industrial toxicants; acrolein; epoxides; 1,2-dihaloethanes
Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
107-02-8; 96-09-3; 75-56-9; 106-93-4; 107-06-2
University of Texas Medical Branch, Galveston, Texas