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Carrageenan stimulates reduction of nitroblue tetrazolium by human neutrophils without membrane depolarization, myeloperoxidase secretion, or increased oxygen consumption.

Authors
Brestel-EP; McClain-EJ; Castranova-V
Source
Inflammation 1986 Dec; 10(4):425-434
NIOSHTIC No.
00167931
Abstract
The mode of action of carrageenan (9000071), a sulfated polyanionic polysaccharide, was investigated in human neutrophils (PMN), in a serum free medium. Carrageenan is an inflammatory agent that is commonly used to test antiinflammatory drugs. PMNs were isolated from citrated whole human blood by countercurrent elutriation and were used for in-vitro experiments, in which they were stimulated by carrageenan and tested for several parameters. Included in the criteria tested were, their effect on nitroblue-tetrazolium (NBT) reduction, oxygen consumption, hydrogen-peroxide reduction, luminol dependent chemiluminescence, release of myeloperoxidase and lactate- dehydrogenase, and membrane depolarization. Carrageenan stimulated the reduction of NBT by PMNs, but did not stimulate membrane depolarization, oxygen consumption, hydrogen-peroxide production, or myeloperoxidase secretion. The authors conclude that carrageenan stimulates a heat labile, NBT reducing system, a mechanism different from that of other PMN activators.
Keywords
NIOSH-Author; Leukocytes; Blood-cells; Immune-reaction; Immune-system; Hemoproteins; In-vitro-studies; Humans
CODEN
INFLD4
CAS No.
9000-07-1
Publication Date
19861201
Document Type
Journal Article
Fiscal Year
1987
Issue of Publication
4
ISSN
0360-3997
NIOSH Division
ALOSH
Source Name
Inflammation
State
WV
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