A comparison of baseline and cyclophosphamide-induced sister chromatid exchanges in bone marrow and spleen cells of mouse and chinese hamster.
Krishna G; Nath J; Petersen M; Ong T
Environ Mutagen 1986 May; 8(3):449-459
Male CD1-mice and male Chinese-hamsters were used to compare the frequencies of sister chromatid exchanges induced by cyclophosphamide (50180) (CY) in the bone marrow and in the spleen. Tests were also performed to validate newly developed rodent bone marrow and lymphocyte cell cultures intended for genotoxicity studies. Baseline studies revealed significant differences in sister chromatid exchange frequencies between the spleens of mice and hamsters and between cell types in hamsters only; the differential between the two species for both cell types was also significant when expressed per chromosome. Following intraperitoneal injection of CY in doses of 10 or 40mg/kg, the sister chromatid exchange levels in cultured bone marrow and spleen cells of hamsters were significantly different from those recorded for mice, both in terms of DNA content and per chromosome. In both animal species, spleen cells were affected to a greater degree in this respect by the drug than were bone marrow cells. The effect of CY was dose dependent. The cell replication index for treated cells was not significantly different from that of controls for both species, regardless of the dose of CY. The authors conclude that the bioassay methods used in this study may be useful for comparative cytogenetic studies in mammals.
NIOSH-Author; Chromosome-damage; Chromosome-disorders; Laboratory-animals; Antineoplastic-agents; Genetic-disorders; Nucleic-acids; Cytotoxicity; In-vivo-studies