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In-depth survey report: control technology assessment of enzyme fermentation processes at Gist-Brocades USA, Inc., Kingstree, South Carolina.
Martinez KF; Sheehy JW
Cincinnati, OH: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, ECTB 116-19b, 1986 Oct; :1-35
The controls and containment capabilities of the proteolytic enzyme manufacturing process was examined at the Gist-Brocades USA, Inc. facility (SIC-2869) located in Kingstree, South Carolina where 160 workers were involved in the production of the industrial enzyme protease (9001927) using a microbial strain of Bacillus-subtilis. A comparison between viable sample concentrations at selected sites and background levels indicated that most emissions occur at high energy operations including the agitator shafts, belt conveyor, filter press, and sampling ports. Exhaust gases from the fermentor and seed tanks were also sites of increased emission levels, as the water scrubber did not completely control the release of entrained production microorganisms. During removal of the filter cake from the filter press, the microbial levels ranged as high as 28990 colony forming units/cubic meter in a single sample. In all but one location of the enzyme recovery area, acetone (67641) levels were less than 0.01mg/m3. According to the authors, emissions of viables are most likely to occur at high energy operations where there is probable aerosolization. Exhaust gases from the fermentor and seed tanks are other sources of emissions; microorganism release is not completely controlled by the water scrubber. Emissions from sampling ports could be lowered by following the company procedure for opening the steam valve.
NIOSH-Author; NIOSH-Survey; Field-Study; Region-4; Enzymes; Antibiotics-industry; Drugs; Pharmaceutical-industry
Field Studies; Control Technology
NTIS Accession No.
National Institute for Occupational Safety and Health
Page last reviewed: May 5, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division