Comparative in vivo and in vitro sister chromatid exchange studies in chinese hamster bone marrow and spleen cells.
Krishna-G; Nath-J; Ong-T
Teratog, Carcinog, Mutagen 1986 Jan; 6(4):321-330
Bone marrow and spleen cells from Chinese hamsters were chosen as models for in-vivo and in-vivo/in-vitro cyclophosphamide (50180) induced sister chromatid exchange (SCE) comparison. Cyclophosphamide was dissolved in phosphate buffered saline and immediately injected intraperitoneally at doses of 10 through 40mg/kg. Cyclophosphamide caused dose related increases in SCEs in the cells under both in-vivo and in-vivo/in-vitro conditions. While the sensitivity of bone marrow and spleen cells to the effects of cyclophosphamide was similar in-vivo, it varied in in-vivo/in-vitro and was higher in spleen cells. Whether or not in-vivo/in-vitro studies can be used to predict in-vivo damage remains to be established by additional studies. Trinitrofluorenone (25322149), a direct acting mutagen, caused equivalent dose related increases in SCEs in both bone marrow and spleen cells in-vitro. These cell types, therefore, can be used as genotoxicity assays under both in- vivo/in-vitro and in-vitro conditions. These systems can be potentially useful in comparative in-vivo and in-vitro cytogenetic studies with same tissues to better assess the genotoxic risk of chemicals.
NIOSH-Author; Laboratory-animals; Chromosome-damage; Chromosome-disorders; Cell-cultures; Cell-metabolism; Antineoplastic-agents; Spleen-disorders; Amides;
Author Keywords: Chinese hamsters; sister chromatid exchanges; primary cell culture; bone marrow; spleen; cyclophosphamide; trinitrofluorenone
Teratogenesis, Carcinogenesis, and Mutagenesis