In vivo and in vitro evaluations of spermatotoxicity induced by 2-ethoxyethanol treatment.
Toxicol Appl Pharmacol 1986 Jul; 84(3):576-583
The mechanism of 2-ethoxyethanol (110805) (EE) action on male reproductive system was studied in-vivo and in-vitro. Male Long- Evans hooded rats were given 0 or 936 milligram/kilogram of EE by gavage, 5 days/week for 6 weeks. The initial sperm count was 60 million. The count decreased 30 to 40 percent after 5 weeks. By the sixth week, three of ten treated rats were azoospermic and the remaining rats had sperm counts between 5 and 30 million. Test animals showed abnormal sperm morphology, but had about the same motility as the control group. Testes, epididymides, and caudae epididymides weights were lower in the treated animals. The treatment of spermatocytes with ethoxyacetic-acid (627032) (EAA), the reported active metabolite of EE, elicited a significant increase in the lactate:endogeneous ratio and a significant decrease in the 2,4-dinitrophenol:lactate ratio, indicating an enhanced utilization of oxygen by the treated spermatocytes. The simultaneous decrease in the 2,4-dinitrophenol:lactate ratio suggested that spermatocytes were at least partially in an uncoupled oxidative state. The EAA treated cells also showed a large decrease in ATP concentration. The authors concluded the results indicate that EAA interferes with energy metabolism in the pachytene spermatocytes which may partially explain the testicular toxicity.
NIOSH-Publication; NIOSH-Grant; Reproductive-system-disorders; In-vivo-studies; In-vitro-studies; Rodents; Testes; Reproductive-hazards; Enzymes; Ethers; Alcohols; Aliphatic-compounds
Environmental Health University of Cincinnati 3223 Eden Avenue Cincinnati, Ohio 45267
Toxicology and Applied Pharmacology
University of Cincinnati, Cincinnati, Ohio