Effects of paternal ethylene dibromide exposure on F1 generation behavior in the rat.
Fanini-D; Legator-MS; Adams-PM
Mutat Res 1984 Mar; 139(3):133-138
The effects of paternal ethylene-dibromide (106934) (EDB) exposure on F1 generation behavior were studied in rats. Adult male Fischer- 344-rats were injected intraperitoneally with 0, 1.25, 2.5, 5.0, or 10.0 milligrams per kilogram EDB daily for 5 consecutive days, then were mated with untreated virgin females 4 and 9 weeks after the last injection. The breeding schedule was followed in order to compare a mid/stage (spermatocyte) with an early germ stage (spermatogonia). After delivery, litter size was recorded. The offspring were weighed. Behavioral assessment of motor reflexes and motor coordination such as swimming performance, open field activity, and surface righting and negative geotaxis were examined up to 21 days of age. There were no significant differences in litter size and neonatal body weight between the control and EDB treated animals at any of the dose/breeding time combinations. Paternal EDB exposure did not significantly alter surface righting and negative geotaxis. Swimming direction and limb movement were significantly impaired in offspring of males bred 4 weeks after exposure. Fewer offspring of males bred 9 weeks after EDB exposure swam with their heads elevated. The amount of ambulation in the open field was significantly suppressed in offspring of EDB males regardless of breeding time. The authors suggest that premeiotic stages of rat spermatogonia are sensitive to the genotoxic effects of EDB. Behavioral assessments of developing F1 offspring from an EDB exposed male are capable of detecting effects induced during spermatogenesis and transmitted through the sperm.
NIOSH-Publication; NIOSH-Grant; Exposure-limits; Molecular-structure; Laboratory-animals; Analytical-models; Acute-toxicity; Environmental-contamination; Molecular-biology; Analytical-instruments; Exposure-levels
Psychiatry and Behavioral Scis University of Texas Dept-Psychiatry & Behav Scis Galveston, Tex 77550
University of Texas Med BR Galveston, Galveston, Texas