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Ethoxyethanol behavioral teratology in rats.
Nelson-BK; Brightwell-WS; Setzer-JV; Taylor-BJ; Hornung-RW; O'Donohue-TL
Neurotoxicology 1981 Oct; 2(2):231-249
Behavioral changes caused by exposure to 2-ethoxyethanol (110805) during gestation were examined in rats. Pregnant Sprague-Dawley- rats were assigned to one of four groups during various gestation periods: 100 parts per million (ppm) on days 7 to 13; 100ppm on days 14 to 20; and sham exposed on days 7 to 13 or 14 to 20. Exposures were conducted 7 hours per day in an exposure chamber. Individual pups were weighed weekly on days 7, 14, 21, 28, and 35 of age and observed for abnormalities. Six behavioral tests were selected to measure various central nervous system functions at several stages of development: ascent, rotorod, open field, activity wheel, avoidance conditioning, and operant conditioning. Tests were selected to evaluate motor, sensory, and cognitive function. Ten pups per treatment group were used for neurochemical analyses. Newborn pups were irradiated at 3.5 kiloWatts for 0.75 second and 21 day old pups were irradiated for 1.0 second. Chemical analyses were performed on whole brain samples from newborn animals; cerebrum, cerebellum, brainstem, and midbrain samples were taken from 21 day old animals. Birth weights did not differ significantly among groups. Offspring from dams exposed on gestation days 7 to 13 had significantly lower mean revolutions per minute for the rotorod test; offspring from rats exposed on gestation days 14 to 20 were marginally less active than those exposed on days 7 to 13 for the open field test. Offspring of rats exposed on gestation days 14 to 20 were less active than controls on the activity wheel. No differences were observed among groups analyzed for operant conditioning or ascent. Norepinephrine concentrations exceeded control values in cerebrum, brainstem, and midbrain of pups exposed on days 7 to 13. The authors conclude that 2-ethoxyethanol produces a number of behavioral and neurochemical deviations in the offspring of rats exposed on gestation days 7 to 13 or 14 to 20.
NIOSH-Author; Laboratory-animals; Brain-function; Neurotoxic-effects; Toxic-effects; Exposure-levels; Chemical-composition; Behavioral-disorders; Teratogens; Laboratory-techniques; Biological-monitoring
Issue of Publication
Neurotoxic Disorders; Neurotoxic-effects
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Content source: National Institute for Occupational Safety and Health Education and Information Division