Lead exposure, begun in utero, decreases renin and angiotensin II in adult rats.
Victery-W; Vander-AJ; Markel-H; Katzman-L; Shulak-JM; Germain-C
Proc Soc Exp Biol Med 1982 May; 170(1):63-67
The effects of in-utero exposure to lead (7439921) on the renin/angiotensin system were studied in adult rats. Male Charles- River-rats were continuously exposed to lead in-utero and after birth by giving their mothers, during pregnancy and lactation, drinking water containing 0, 5, or 25 parts per million (ppm) lead- acetate and then continuing this protocol after weaning for approximately 5 months. Blood pressure recordings were obtained when the animals reached 2 months of age, again at 3 months, and weekly thereafter. The animals were killed 21 weeks after weaning, and trunk blood was collected and assayed for blood lead, plasma renin activity and angiotensin II. Three hours prior to killing, half of the animals in each treatment group were injected with polyethylene-glycol. Systolic blood pressures did not differ significantly between treated and control rats at any time during the experiment. Mean blood lead concentrations in animals exposed to 5 and 25ppm lead were 5.6 and 18.2 micrograms per deciliter, respectively. Mean basal plasma renin activity was significantly lower in animals given 25ppm lead-acetate than in the controls. Mean plasma renin activity in rats dosed with 25ppm lead-acetate and given polyethylene-glycol just before killing was significantly elevated. The ratio of angiotensin II to plasma renin activity in rats given 5 or 25ppm lead-acetate and polyethylene-glycol was significantly decreased. The authors conclude that lead causes an inhibition of angiotensin II generation or an enhancement of angiotensin II breakdown. The lowest dose of lead producing this effect lies between 5 and 25ppm. This data suggests a need for evaluating angiotensin II/plasma renin activity in lead exposed individuals since a change in this ratio might be a sensitive indicator of biologically important lead effects.
NIOSH-Publication; NIOSH-Grant; Physiological-testing; Quantitative-analysis; Chemical-composition; Laboratory-animals; Dose-response; Blood-analysis; Biological-effects; Bioassays; Exposure-limits
Other Occupational Concerns; Grants-other
Proceedings of the Society for Experimental Biology and Medicine
University of Michigan at Ann Arbor, Ann Arbor, Michigan