Chemical permeation at specific glove sites was studied. The thickness at 23 specific sites of five latex neoprene gloves of the same lot were measured using a dial gauge. Gloves were turned inside out and filled with 100 milliliters of acetone. Glove necks were tied and the tied necks were evaluated with a photoionization detector for sealing ability. The detector measured the glove sites for acetone concentration. Breakthrough time was calculated. Specific site breakthrough times were normalized for minutes per square millimeter. Mean breakthrough times were calculated. The mean acetone concentration under steady state equilibrium at each of the specific sites was calculated by averaging four concentrations at different time intervals greater than 34 minutes. Specific site breakthrough times were statistically different, but site specific normalized breakthrough times were equivalent. Mean breakthrough times were less than 16 minutes at the thinnest sites, between the fingers, the back, and the palm, 16 to 21 minutes at intermediate sites of the sides of fingers and glove, and over 21 minutes at the thickest sites, the fingertips. The mean steady state concentration was largest at the thinnest specific sites. The authors conclude that appropriate glove sites for liquid chemical permeation testing are the palm and the back of the glove.