Correlative genotoxicity studies of airborne particles in Salmonella typhimurium and cultured human lymphocytes.
Krishna-G; Nath-J; Ong-T
Environ Mutagen 1984 Jan; 6(4):585-592
The effects of solvent air particulates on genetic alterations were examined in human cells. Airborne particles were collected using high purity glass microfiber filters. The sample filters were soaked in acetone, after which the sample was filtered and the acetone extract obtained was concentrated. The dried extracts were dissolved in reagent grade dimethylsulfoxide (DMSO). Heparinized peripheral lymphocytes from two normal human donors were cultured in the dark at 37 degrees-C for 72 hours in culture medium for use in the performance of a sister chromatid exchange (SCE) assay. In order to perform a chromosomal aberration assay, human peripheral blood was cultured in an identical manner for 52 hours. The same organic airborne particle extract concentrations were tested in Salmonella-typhimurium strains TA-98 and TA-100 without metabolic activation for an Ames Salmonella assay. The Student's t-test was used to compare SCE and histidine reversion frequencies to negative controls DMSO. Data indicated clear dose responses. At the highest concentration, 10.93 milligrams (mg) of particulates per 10 milliliters (ml) of culture, the average SCE frequency was about 3 times greater than the background value. Comparable responses were obtained in blood donors at all concentrations. The combined aberration frequency also exhibited a dose related response, with 13 chromosomal aberrations per 100 cells being observed at 10.93mg/10ml. A dose related response was also observed at the same concentrations for gene mutations. The authors recommend that the SCE analysis of human peripheral lymphocytes be employed in future genotoxicity studies related to ambient air particulates, as well as other complex mixtures found in the environment.
NIOSH-Author; Medical-research; Airborne-particles; Bioassays; Qualitative-analysis; Chromosome-damage; Cell-damage; Cytotoxic-effects; Dose-response; Biological-effects; Respirable-dust; Dust-analysis