A primate model of byssinosis: pulmonary changes following inhalation of cotton dust extract.
Ainsworth-SK; Moorman-W; Mundie-TG; Pilia-PA; Bishop-MP; Lewis-TR
Cotton dust: proceedings of the Eighth Cotton Dust Research Conference, beltwide cotton production research conferences, January 9-10, 1984, Atlanta, Georgia. Wakelyn PJ, Jacobs RR, eds. Memphis, TN: The National Cotton Council of America, 1984 Jan; :87-91
Pulmonary function was studied in monkeys after acute and chronic exposure to cotton dust extracts. Male cynomolgus-monkeys were anesthetized and endotracheally intubated for measurement of pulmonary mechanics. Bronchoprovocation challenges were performed with cotton dust extracts at 0.01 gram per milliliter and acetyl-B-methylcholine. Responses to 30 breaths of either agent were compared with baseline responses to 30 breaths of saline. The immediate change response at 10 minutes and at 2 hours was studied. Animals were further exposed twice weekly for 3 months. Immediate acute response consisted of slight increases of baseline value for resistance, at 120 to 130 percent, and very slight decrease in flow performance of 2 to 3 percent. At 2 hours responses increased, indicating some time was required for development of change. Two of eight monkeys were non responders. Four of the eight were classified as definite responders with either a 200 percent increase in resistance or an 80 percent decrease in forced expiratory flow after acute challenge. Half of the monkeys demonstrating a preexposure acute response became tolerant to the cotton dust extract challenge after chronic exposure. Methacholine responsiveness increased. Two preexposure responders became even more responsive after long term challenge. The authors conclude that the monkey is an excellent model for the study of byssinosis because the variability of response to bronchoprovocation with cotton dusts parallels that of humans.
Animal-studies; Pulmonary-function; Pulmonary-system; Respiration; Inhalants; Laboratory-techniques; Respirators; Analytical-methods; Monitoring-systems; Breathing
Cotton dust: proceedings of the Eighth Cotton Dust Research Conference, beltwide cotton production research conferences, January 9-10, 1984, Atlanta, Georgia