Genotoxic activity of nitrosated coal dust extract in mammalian systems.
Tucker-JD; Whong-Z; Xu-J; Ong-M
Environ Res 1984 Oct; 35(1):171-179
The mutagenic capability of nitrosated coal dust extract was examined in-vitro in mice. Subbituminous coal was extracted with dichloromethane, methanol, and acetone before being nitrosated with sodium-nitrite (7632000). The nitrosated coal extract was added to human peripheral lymphocyte cultures and at 48 hours the frequency of sister chromatid exchange (SCE) was measured. A Chinese-hamster ovary culture was treated in a similar manner. Male Swiss-Webster- mice were given 0.15 milliliter per 10 grams of the test compound by oral gavage. Mouse bone marrow cells were assayed for micronucleated cells. Nitrosated coal dust extract produced an elevated SCE frequency among human lymphocytes. Chinese-hamster ovary cells also had an elevated SCE frequency after growing in the presence of nitrosated coal dust extract. Both cell lines had chromosomal aberrations; cell cycle delay was also apparent. Non nitrosated extract was toxic for erythrocytes but nitrosated extract was not. Mouse lymphoma cells had an elevated mutational frequency. The authors conclude that nitrosated coal dust extract is genetically active in-vitro without metabolic activation in mammalian assay systems.
NIOSH-Author; Bioassays; Stomach-cancer; Industrial-dusts; Qualitative-analysis; Mutagenicity; Aerosol-particles; Airborne-particles; Coal-miners; Occupational-diseases; Occupational-medicine; Medical-research