Accumulation of lead by renal slices in the presence of organic anions.
Proc Soc Exp Biol Med 1981 Apr; 166(4):583-586
The effects of the addition of organic anions on the accumulation of lead-203 (14687253) (Pb-203) were investigated in renal slices from rabbits. Rabbits were killed and renal cortical slices were prepared. Slices were preincubated for 30 minutes in Krebs Ringer bicarbonate solution or Tris buffer solution, then transferred to flasks containing various concentrations of cysteine, citrate, cystine, glutathione, bovine serum albumin, histidine, or ultrafiltrable rabbit serum, together with lead-acetate and Pb-203 labeled lead-acetate. No differences were observed among the buffer solutions. Accumulation of Pb-203 was markedly reduced by the addition of cysteine. The renal slice to medium ratio was significantly increased in the presence of cysteine or citrate, but was not affected by cystine or serum ultrafiltration, and no obvious effects were seen with glutathione or histidine. Addition of sodium- cyanide or sodium-iodoacetate significantly reduced the ratios for Pb-203 when only the basic protein containing medium was used; the mass of Pb-203 accumulated by the slices was not altered by the inhibitors. The authors conclude that lead accumulation by renal slices is by free ion transport and that transport with low molecular weight ligands plays at most a minor role. The lack of alteration of the slice/medium ratio by metabolic inhibitors supports the hypothesis that the ratio is due to lead binding by plasma membranes and not to actual transport into the cells.
NIOSH-Publication; NIOSH-Grant; In-vitro-study; Body-retention; Radiochemical-analysis; Pharmacodynamics; Medical-research; Histochemical-analysis; Toxicopathology; Renal-toxicity; Molecular-biology
Physiology University of Michigan 6811 Medical Science II Ann Arbor, Mich
Other Occupational Concerns; Grants-other
Proceedings of the Society for Experimental Biology and Medicine
University of Michigan at Ann Arbor, Ann Arbor, Michigan