Ten NIOSH Analytical Methods - ANTU.
NIOSH 1979 Apr:21 pages
The NIOSH validated method for the determination of alpha- naphthylthiourea (86884) (ANTU) is described. Four hundred eighty liters of air (flow rate at 1.5 to 2.0 liters per minute) is drawn through a 37 millimeter diameter teflon filter to collect the ANTU. The filter is transferred to a 20 milliliter (ml) vial and extracted with 10ml of methanol. Ten microliters of the solution are injected into a high pressure liquid chromatograph. The area of the sample peak is determined. The concentration of ANTU is determined by comparison with a calibration curve obtained by analyzing standards having concentrations of 0.1 to 3 times the OSHA standard of 0.3 milligrams per cubic meter (mg/m3). Storage stability of the samples is determined by analyzing a standard containing 0.293mg/m3 ANTU at 0 and 7 days after collection. The concentrations are 0.299 and 0.295mg/m3 after 0 and 7 days, respectively. The method is suitable for determining concentrations of 0.128 to 0.76mg/m3 with a standard deviation of 0.016mg/m3. The detection limit is estimated to be 5 micrograms of ANTU per sample filter.
NIOSH-Author; NIOSH-Analytical-Method; Analytical-Method; Standards; Sampling; Standards-Completion-Program; SCP; Set-7; Chromatographic-analysis; Thioureas;
NTIS Accession No.
Division of Physical Sciences and Engineering, NIOSH, U.S. Department of Health and Human Services, Cincinnati, Ohio, 21 pages, 4 references