In Vivo Effects Of Metals On Lung Peroxidation, Final Report.
The in-vitro effects of metals on lung lipid peroxidation were examined. Guinea-pig or rat lung microsomes were incubated with iron (7439896), zinc (7440666), cadmium (7440439), nickel (7440020), cobalt (7440484), tin (7440315), manganese (7439965), or lead (7439921) and assayed for evidence of lipid peroxidation. Only iron caused lipid peroxidation. The lung microsomes were then incubated with each of the metals in combination with iron. Cadmium and zinc enhanced the iron induced lipid peroxidation. Cobalt, nickel, tin, and manganese inhibited iron induced lipid peroxidation. The other metals had no effect. Lung microsomes were assayed for glutathione, superoxide-dismutase, glutathione-peroxidase, ascorbate, and glutathione-reductase. All substances were found to be present in measurable quantities. Their protective abilities against lipid peroxidation were measured. Only ascorbate was found to protect the lungs against lipid peroxidation. The authors note that an active transport system for ascorbate exists in lung cells. This ensures the availability of ascorbate for its protective function. Results of in-vitro studies of metal combinations on lipid peroxidation are important since occupational exposures usually involve combinations of metals rather than individual metals.
NIOSH-Author; Heavy-metals; Toxic-effects; Bioassays; Enzymatic-effects; Industrial-medicine; Occupational-medicine; Biological-effects; Cytotoxic-effects;
7439-89-6; 7440-66-6; 7440-43-9; 7440-02-0; 7440-48-4; 7440-31-5; 7439-96-5; 7439-92-1;
NTIS Accession No.
NIOSH, U.S. Department of Health, Education, and Welfare, Cincinnati, Ohio, 4 pages, 9 references