NIOSH 1978 Oct:30 pages
An analytical method was developed for the determination of airborne concentrations of strychnine (57249). A known volume of air was drawn through a glass fiber filter to trap strychnine particulates. Filters were extracted with an ion pairing reagent and solutions were analyzed by high pressure liquid chromatography using an ultraviolet detector set at 254 nanometers. The method was validated over a range of 0.0730 to 0.343 milligrams per cubic meter (mg/m3) using a 180 liter air sample. Validation of the method consisted of analysis of 18 spiked samples containing 13.52, 27.04, and 54.1 micrograms of strychnine (representing 50, 100, and 200 percent of the OSHA standard of 0.15mg/m3) and the analysis of 18 samples of each of the concentrations collected from dynamically generated test atmospheres. Collection efficiency, storage stability, and precision and accuracy were measured. The total coefficient of variation was 0.059, and collection efficiency was 98.8 percent. The samples were stable when stored for 7 days. The authors conclude that the advantage of the method is that the sampling device is small, portable, and involves no liquids. Interferences are minimal. The method is adequate for sampling and analysis of strychnine as the neutral base or its salt.
NIOSH-Author; NIOSH-Analytical-Method; Analytical-Method; Standards; Sampling; Standards-Completion-Program; SCP; Set-6; Chemical-analysis;
NTIS Accession No.
Ten NIOSH Analytical Methods, Set-6, Division of Physical Sciences and Engineering, NIOSH, Cincinnati, Ohio, NTIS PB288-629, 30 pages, 6 references