Screening tests of priority chemicals for possible reproductive hazards were conducted. Fifteen compounds which included phthalate esters, aromatic amines, and organophosphates were used. Each compound was administered orally to female CD1-mice at 10 milliliters per kilogram daily for 8 days. Minimum effective doses (MED) were calculated. The MED was defined as the highest dose that caused a small number of deaths or significant weight loss. The MED of each compound was administered on days 7 through 14 of gestation. Clinical observations were made and necropsies were conducted. Mean body weights were obtained daily. Litter size, number of live pups, body weight, and body weight changes were recorded and statistically analyzed. Results indicated that di(n-butyl)phthalate (84742), di(isobutyl)phthalate (84695), N-isopropyl-N'-phenyl-p- phenylenediamine (101724), di(2-ethylhexyl)phthalate (117817), 2- ethyl-1-hexanol (104767), di(n-hexyl)phthalate (84753), and mono(2- ethylhexyl)phthalate (4376209) would require high priority consideration for more detailed reproductive toxicity testing. Diethyl-phthalate (84662), dimethyl-methylphosphonate (756796), tris(2-chloroethyl)phosphate (115968), d-phenylalanine (673063), probenecid (57669), trans-cinnamaldehyde (14371109), di(isodecyl)phthalate (26761400), and di(n-octyl)phthalate were found to warrant lower priority for detailed reproductive toxicity studies.
NIOSH, Centers for Disease Control, U.S. Department of Health and Human Services, Atlanta, Georgia